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KMID : 0364820140500020152
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Korean Journal of Microbiology
2014 Volume.50 No. 2 p.152 ~ p.157
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Phylogenetic Diversity of Bacterial Community Inhabited in Callyspongia elegans
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Park So-Hyun
Kim Ji-Young
Kim Young-Ju
Heo Moon-Soo
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Abstract
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The aim of this study was to investigate the bacterial community inhabited in Callyspongia elegans. Marine bacteria
were isolated from the marine sponge C. elegans using marine agar. The resulting 112 isolated pure cultures were
then used for further study. They were characterized by determining morphological characteristics through Gram’s
staining and morphological observation. The colony pigments of bacterial isolates were characterized as yellow,
brown, ivory, and white. Thirty-seven strains were found to be Gram-positive and 75 strains were Gram-negative.
Seventy-nine strains were coccus-shaped, while 16 strains were rod-shaped. On the basis of the results of the
comparative analyses of 16S rDNA gene sequences, the 112 isolated bacteria were divided into 5 major groups:
Alphaproteobacteria (39%), Gammaproteobacteria (22%), Actinobacteria (14%), Fimicutes (9%), and Bacteroidetes
(6%). It is strongly suggested that fifteen isolates are candidates for a new genera or species, based on the analyses
of 16S rDNA gene sequences.
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KEYWORD
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16S rDNA, Callyspongia elegans, bacterial community, marine sponge, phylogenetic tree
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